Journal
COMPTES RENDUS PHYSIQUE
Volume 3, Issue 4, Pages 439-448Publisher
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/S1631-0705(02)01329-4
Keywords
NLO-phores; fluorophores; two-photon absorption; TPEF; SHG; molecular probes; cell membranes
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Novel microscopies based on nonlinear optical (NLO) phenomena such as two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) have gained overwhelming popularity in the biology community owing to the many advantages they provide in biological imaging. Examples of molecular engineering approaches toward NLO-probes specifically designed for SHG and/or TPEF imaging of lipid membranes and biological cells are given here, providing an illustration of their intriguing potential in the area of real-time. non-damaging imaging of biological structures. Optimized NLO-markers open new routes for improved monitoring and better understanding of fundamental dynamic processes. (C) 2002 Academie des sciences/Editions scientifiques et medicales Elsevier SAS.
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