Journal
BIOCONJUGATE CHEMISTRY
Volume 13, Issue 3, Pages 387-391Publisher
AMER CHEMICAL SOC
DOI: 10.1021/BC015558q
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Funding
- NIGMS NIH HHS [GM57464, R01 GM057464] Funding Source: Medline
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The cyanine dyes Cy3 and Cy5 have proven valuable in numerous applications involving conjugation with proteins. Practical syntheses of lysine-selective, succinimidyl ester derivatives of these dyes have been published, and succinimidyl esters are commercially available. However, the published syntheses of cysteine-selective derivatives produce relatively low yields from expensive starting materials, or produce molecules with marginal water solubility for protein labeling. We report here facile syntheses (four steps, >50% overall yield) of iodoacetamide, sulfhydryl-reactive derivatives of the Cy3 and Cy5 fluorophores. These novel derivatives have good water solubility (>2.5 mM) and bear only one reactive side chain, reducing possible protein cross-linking encountered with previous derivatives.
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