Journal
JOURNAL OF PROTEOME RESEARCH
Volume 1, Issue 3, Pages 233-237Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr025506q
Keywords
microarray; ELISA; breast cancer; hepatocyte growth factor
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We developed an ELISA in high-density microarray format to detect hepatocyte growth factor (HGF) in human serum. The microassay can detect HGF at sub-pg/mL concentrations in sample volumes of 100 muL or less. The microassay is also quantitative and was used to detect elevated HGF levels in sera from recurrent breast cancer patients. The microarray format provides the potential for high-throughput quantitation of multiple biomarkers in parallel, as demonstrated with a multiplex analysis of five biomarker proteins.
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