Journal
PLANT AND CELL PHYSIOLOGY
Volume 43, Issue 5, Pages 513-521Publisher
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcf061
Keywords
cyanobacterium; motility; pilus biogenesis; split cheA; Synechocystis sp PCC 6803; transformation competency
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The unicellular motile cyanobacterium Synechocystis sp. PCC 6803 exhibits phototactic motility that depends on the type 1 like thick pilus structure. By gene disruption analysis, we showed that a gene cluster of slr1041, slr1042, slr1043 and slr1044, whose predicted products are homologous to PatA, CheY, CheW and MCP, respectively, was more or less required for pilus assembly, motility and natural transformation competency with extraneous DNA. By sequence homology, the missing cheA-like gene in this cluster was identified as novel split genes, slr0073 and slr0322, at separate loci on the genome. This was confirmed by nonmotile phenotype of their disruptants. Unique hyperpiliation was observed in the slr1042 and slr0073 disruptants, suggestive of their specific interaction with pilT1. The genes, thus identified as pil genes in this study, were designated piIG (slr1041), pilH (slr1042), pill (slr1043), pilJ (slr1044), pilL-N (slr0073) and pilL-C (slr0322).
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