Journal
IMMUNITY
Volume 16, Issue 5, Pages 707-718Publisher
CELL PRESS
DOI: 10.1016/S1074-7613(02)00312-6
Keywords
-
Categories
Ask authors/readers for more resources
Editing of autoreactive antigen receptors by secondary V(D)J recombination efficiently rescues B lymphocyte precursors from apoptosis induced by negative selection, but its role has not been rigorously assessed in T cell development. We therefore generated a transgenic mouse model in which self-reactive thymocytes could edit their TCR by secondary recombination at the TCRalpha locus. For this purpose, the ValphaJalpha exon of a male-specific TCR was inserted into the TCRalpha. locus followed by Cre-IoxP-mediated deletion of the TCRdelta locus. In this model, only few thymocytes escaped negative selection by change of specificity, probably through recombination before encounter of autoantigen. In the absence of the restricting MHC element, however, developing thymocytes replaced the inserted TCRa exon efficiently.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available