Journal
JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY
Volume 61, Issue 5, Pages 384-395Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/jnen/61.5.384
Keywords
Alzheimer disease (AD); neurofibrillary tangle (NFT), NFT-tree; phosphorylation; synaptophysin; tau
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Funding
- NIA NIH HHS [R01 AG01121, AG09016, AG08665, 5T32 AG00107] Funding Source: Medline
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The data presented here examine 2 hypotheses: 1) that viable but vulnerable single neurons remaining in the Alzheimer brain lose synaptic markers. and 2) that the extent of this loss is related to the disease,rate of these single neurons when disease state is defined by immunoreactivity. We used double immunohistochemistry (IHC) to define neurofibrillary tangle (NFT) and phosphorylation status of tau at selected defined epitopes. This double IHC was combined with quantitative in situ hybridization for message for the synaptic marker, synaptophysin. in 1.127 single hippocampal CA1 pyramidal neurons from 15 Alzheimer disease (AD) and 4 control cases, We found that there is a graded. progressive, decrease of synaptophysin message expressed by single neurons related to immunohistochemical markers Of tau Status, and that neurons in similar immunohistochemically defined classes show similar losses of synaptophysin message regardless of whether they were sampled from clinical control brains or advanced AD, The resulting conclusions are consistent with U suggestion that differences among clinically defined AD and control status are defined by the numbers of neurons in various disease states.
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