Journal
NEUROREPORT
Volume 13, Issue 6, Pages 909-915Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/00001756-200205070-00035
Keywords
EGF motif; glycosylation; neural network; olfactory bulb; Purkinje cells; pyramidal cells; transmembrane protein
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Using the signal sequence trap method, we have identified a cDNA clone coding for a type I transmembrane protein, BET, with 10 epidermal growth factor (EGF) motifs in the extracellular domain. In situ hybridization revealed that the bet m RNA is specifically expressed in the mitral/tufted cells in the olfactory bulb, Purkinje cells in the cerebellum, and pyramidal cells in the hippocampus. Using polyclonal antibodies, we have demonstrated that the BET protein is highly glycosylated and is localized in patches in the dendrites and in the somata of neurons. Since the predicted structure of BET shares many similarities with the Notch ligands, this novel protein may play crucial roles in establishing the neuronal networks in the olfactory system, cerebellum, and hippocampus. BET is the first transmembrane protein containing only multiple EGF-like repeats specifically expressed in the brain. NeuroReport 13:909-915 (C) 2002 Lippincott Williams Wilkins.
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