Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 124, Issue 18, Pages 4980-4986Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ja0123608
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Funding
- NIGMS NIH HHS [R01 GM051105, GM 51105] Funding Source: Medline
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Backbone cyclization is often used in attempts to enhance protein stability, but is not always successful as it is possible to remove stabilizing or introduce destabilizing interactions in the process. Cyclization of the PIN1 WW domain, a 34-residue three-stranded beta-sheet structure, removes a favorable electrostatic interaction between its termini. Nevertheless, optimization of the linker connecting the N- and C-termini using information based on the previously determined ensemble of NMR structures leads to beta-sheets that are more stable than those derived from the linear sequence. Linkers that are too short or too long introduce strain, likely disrupting native interactions, leading to cyclic folds that are less stable than that of the linear sequence.
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