Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 955, Issue 2, Pages 245-256Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0021-9673(02)00214-5
Keywords
Fusarium spp.; derivatisation, LC; mycotoxins; zearalenone; trichothecenes; fumonisins
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Various liquid chromatographic methods used in the analysis of mycotoxins (zearalenone, trichothecenes and fumonisins) produced by Fusarium species were compared in this work. The results demonstrate the suitability of modem clean-up procedures employing multi functional MycoSep and immunoaffinity columns although these methods are more expensive than conventional methodologies for clean-up. HPLC with both fluorescence and photodiode array detection is a suitable technique for the analysis of toxic secondary metabolites produced by Fusarium species; different derivatisation strategies have been studied to improve the sensitivity of the technique because of the low concentration of these metabolites in contaminated food. The utility of the proposed methodology was assessed in cereal cultures of various Fusarium strains. (C) 2002 Elsevier Science B.V. All rights reserved.
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