Journal
NEUROSCIENCE LETTERS
Volume 324, Issue 1, Pages 57-60Publisher
ELSEVIER SCI IRELAND LTD
DOI: 10.1016/S0304-3940(02)00172-6
Keywords
14-3-3 protein; 14-3-3 protein capture assay; Creutzfeldt-Jakob disease; prion protein; cerebrospinal fluid
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A protein capture assay was used to measure 14-3-3 (gamma-isoform) in the cerebrospinal fluid (CSF) of patients with either variant or sporadic Creutzfeldt-Jakob disease (CJD). The results were compared with those obtained using Western blotting. Elevated levels of 14-3-3gamma were found in 58% of variant CJD (vCJD) patients and 82% of sporadic CJD (spCJD) patients using the protein capture assay. Using a Western blotting technique, the presence of CSF 14-3-3gamma was detected in 58% of vCJD patients and in 89% of spCJD patients. When the results from the protein capture assay and the Western blot were combined, 14-3-3gamma was detected in 77% of vCJD patients and in 91% of spCJD patients. These results suggest that although analysis of CSF 14-3-3 is not as useful in vCJD as it is in the sporadic form of the disease, a combination of these two techniques results in increased sensitivity of 14-3-3 for the diagnosis of vCJD. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
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