Simultaneous determination of terbinafine HCL and triamcinolone acetonide by UV derivative spectrophotometry and spectrodensitometry

A binary mixture of terbinafine hydrochloride and triamcinolone acetonide was determined by three different methods. The first one concerned with determination of both drugs using first derivative (D,) spectrophotometric technique at 297 and 274 nm over concentration ranges of 5-30 and 4-24 mug ml(-1), with mean percentage accuracies 99.90 +/- 0.67 and 100.25 +/- 0.49, respectively. The second method depends on ratio-spectra 1st derivative (RSD1) spectrophotometry at 298 and 248 nm over the same concentration ranges with mean percentage accuracies 100.22 +/- 0.51 and 99.93 +/- 0.56, respectively. The spectrodensitometric analysis provides a rapid and precise method for the separation and quantitation of both terbinafine hydrochloride and triamcinolone acetonide. The method depends on the quantitative densitometric evaluation of thin layer chromatogram of terbinafine hydrochloride and triamcinolone acetonide at 283 and 238 nm over concentration ranges of 5-25 and 2.5-22.5 mug spot(-1), with mean percentage accuracies 100.66 +/- 0.51 and 100.27 +/- 0.73, respectively. The suggested procedures were checked using laboratory prepared mixtures and were successfully applied for the analysis of their pharmaceutical preparations. The three methods retained their accuracy and precision when applying the standard addition technique. The results obtained by applying the proposed methods were statistically analysed and compared with those obtained by a reported method. (C) 2002 Elsevier Science B.V. All rights reserved.