Journal
NUCLEIC ACIDS RESEARCH
Volume 30, Issue 10, Pages -Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/30.10.e47
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Funding
- Intramural NIH HHS [Z01 AG000767] Funding Source: Medline
- NCI NIH HHS [R29CA69421] Funding Source: Medline
- NIA NIH HHS [AG13478, K01 AG00767] Funding Source: Medline
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It has long been presumed impossible to measure telomeres in vertebrate DNA by PCR amplification with oligonucleotide primers designed to hybridize to the TTAGGG and CCCTAA repeats, because only primer dimer-derived products are expected. Here we present a primer pair that eliminates this problem, allowing simple and rapid measurement of telomeres in a closed tube, fluorescence-based assay. This assay will facilitate investigations of the biology of telomeres and the roles they play in the molecular pathophysiology of diseases and aging.
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