Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 84, Issue 2-3, Pages 283-289Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/S0925-4005(02)00016-3
Keywords
microchannel; PCR; ITO; PS-Na; Escherichia coli HB101
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A microchannel chip for continuous-flow polymerase chain reaction (PCR) was developed using transparent materials. The microchannel was fabricated on a quartz glass substrate using standard photolithography and wet-etching techniques and was sealed by another quartz glass substrate. Two indium-tin-oxide (ITO) films were deposited on the etched substrate as a thermal source. To confirm the temperature distribution in the microchannel, we measured the fluorescence spectra of an aqueous solution of 1-pyrenesulfonic acid sodium salt (PS-Na), which is a temperature-indicator dye, in the microchannel under a continuous solution flow. The results confirm that the temperature distribution on the microchannel's ITO films was almost uniform (within +/-2 degreesC) under two flow rates (56 and 152 nl/min). The slightness of this deviation indicates that the ITO films integrated into the microchannel chip can be very useful as a thermal source for PCR. An amplification of a 450 bp segment of Escherichia coli HB101 was successfully per-formed by two-stage (94 and 67 degreesC) thermal cycling on the chip device. (C) 2002 Elsevier Science B.V. All rights reserved.
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