Journal
FEBS LETTERS
Volume 519, Issue 1-3, Pages 141-146Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(02)02741-2
Keywords
antimicrobial peptides; cathelicidin; intein; expression system; transgenic plant; biotherapeutic
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Tobacco plants were engineered to express SMAP-29, a mammalian antimicrobial peptide of innate immunity, as fusion protein with modified vacuolar membrane ATPase intein. The peptide was purified taking advantage of the intein-mediated self-cleaving mechanism. SMAP-29 was immunologically detected in the chromatographic eluate and appeared tightly bound to copurified plant proteins. Electrophoretic separation under disaggregating conditions indicated that the recombinant peptide was cleaved off by intein at the expected site and an overlay gel assay demonstrated that the peptide retained antimicrobial activity. These results indicate that a modified intein expression system can be used to produce pharmaceutical peptides in transgenic plants. (C) 2002 Published by Elsevier Science B.V.. on behalf of the Federation of European Biochemical Societies.
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