Journal
ORIGINS OF LIFE AND EVOLUTION OF THE BIOSPHERE
Volume 32, Issue 3, Pages 219-224Publisher
KLUWER ACADEMIC PUBL
DOI: 10.1023/A:1016513114799
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The phosphorylation of adenosine with trimetaphosphate in solution, in solid phase and using wet-dry cycles was carried out and it was found that wet-dry cycles were the most efficient. The catalytic effects of some metal ions on the phosphorylation were also studied and it was discovered that Ni(II) is the most effective. The combination of wet-dry cycles (4 cycles) and catalysis by Ni(II) led to an unprecedented high conversion of adenosine to phosphorylated products (30%) near neutral pH. The main phosphorylated products were 2', 3'-cyclic AMP (10.4%) and 5'-ATP (13.0%).
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