Journal
CELLULAR SIGNALLING
Volume 14, Issue 6, Pages 529-536Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/S0898-6568(01)00277-7
Keywords
actin; PKC; actomyosin stress fibres; 3T3 cells
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Proteins coimmunoprecipitating with protein kinase C (PKC) epsilon in fibroblasts were identified through matrix-assisted laser desorption/ionisation time of flight mass spectrometry (MALDI TOF m/s). This method identified myosin IIA in PKC epsilon immunoprecipitates, as well as known PKC epsilon binding proteins, actin, beta' Cop and cytokeratin. Myosin is not a substrate for PKC epsilon. Immunofluorescence analysis showed that PKC epsilon is colocalised with actin and myosin in actomyosin stress fibers in fibroblasts. Inhibitors of PKC epsilon and myosin ATPase activity, as well as microfilament-disrupting drugs, all inhibited spreading of fibroblasts after passage, suggesting a role for a PKC epsilon-actin-myosin complex in cell spreading. (C) 2002 Elsevier Science Inc. All rights reserved.
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