Journal
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
Volume 82, Issue 8, Pages 881-885Publisher
WILEY
DOI: 10.1002/jsfa.1117
Keywords
species identification; PCR-SSCP; clam species; Ruditapes decussatus; Venerupis pullastra
Ask authors/readers for more resources
Genetic differentiation of the clam species Ruditapes decussatus (grooved carpet shell) and Venerupis pullastra (pullet carpet shell) has been achieved based on polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis. A short fragment (150 bp) of the alpha-actin gene was amplified by PCR. Amplicons were denatured to obtain single-stranded DNA, electrophoresed on a non-denaturing polyacrylamide gel and visualised by silver staining for detection of SSCPs. Species-specific DNA band patterns were obtained for R decussatus and V pullastra, allowing clear differentiation of the two clam species. (C) 2002 Society of Chemical Industry.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available