Journal
ANALYTICAL CHEMISTRY
Volume 74, Issue 11, Pages 2451-2457Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac0255330
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Funding
- NHGRI NIH HHS [HG 01642-04] Funding Source: Medline
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We have developed an integrated microfabricated cell sorter using multilayer soft lithography. This integrated cell sorter is incorporated with various microfluidic functionalities, including peristaltic pumps, dampers, switch valves, and input and output wells, to perform cell sorting in a coordinated and automated fashion. The active volume of an actuated valve on this integrated cell sorter can be as small as 1 pL, and the volume of optical interrogation is similar to100 fL. Different algorithms of cell manipulation, including cell trapping, were implemented in these devices. We have also demonstrated sorting and recovery of Escherichia coli cells on the chip.
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