Oxygen exchange in relation to carbon assimilation in water-stressed leaves during photosynthesis

In a study on metabolic consumption of photosynthetic electrons and dissipation of excess light energy under water stress, O-2 and CO2 gas exchange was measured by mass spectrometry in tomato plants using O-18(2) and (CO2)-C-13. Under water stress, gross O-2 evolution (E-o), gross O-2 uptake (U-o), net CO2 uptake (P-N), gross CO2 uptake (TPS), and gross CO2 evolution (E-C) declined. The ratio P-N/E-o fell during stress, while the ratios U-o/E-o and E-c/TPS rose. Mitochondrial respiration in the light, which can be measured directly by (CO2)-C-12 evolution during (CO2)-C-13 uptake at 3000 mul l(-1) (CO2)-C-13, is small in relation to gross CO2 evolution and CO2 release from the glycolate pathway. It is concluded that PSII, the Calvin cycle and mitochondrial respiration are down-regulated under water stress. The percentages of photosynthetic electrons dissipated by CO2 assimilation, photorespiration and the Mehler reaction were calculated: in control leaves more than 50 % of the electrons were consumed in CO2 assimilation, 23 % in photorespiration and 13 % in the Mehler reaction. Under severe stress the percentages of electrons dissipated by CO2 assimilation and the Mehler reaction declined while the percentage of electrons used in photorespiration doubled. The consumption of electrons in photorespiration may reduce the likelihood of damage during water deficit. (C) 2002 Annals of Botany Company.