Journal
JOURNAL OF DENTAL RESEARCH
Volume 81, Issue 6, Pages 395-398Publisher
INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R
DOI: 10.1177/154405910208100608
Keywords
IFN gamma deficiency; Porphyromonas gingivalis; inflammation; cytokines; antibody
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Infection with the periodontal pathogen Porphyromonas gingivalis causes a strong local inflammatory reaction. Using IFN-gamma-deficient mice, we tested the hypothesis that the absence of IFN-gamma would result in a reduction of the local proinflammatory response to P. gingivalis. Cytokine secretion by macrophages from IFNgamma(-/-) animals was significantly attenuated. Addition of IFNgamma restored cytokine secretion. In vivo injection of P. gingivalis into subcutaneous chambers increased the intra-chamber leukocyte counts and TNFalpha and IL-1beta levels. This increase was significantly lower in the IFNgamma(-/-) mice. Local reconstitution of IFN-gamma(-/-) mice at the site of inflammation with the IFN-gamma gene increased the levels of TNFalpha and decreased the IL-10 levels. Anti-P. gingivalis IgG1 levels, a marker of Th2 response, were higher in immunized IFN-gamma(-/-) than in IFN-gamma(+/+) mice. The results suggest that lack of IFNgamma reduced the amplitude of the local pro-inflammatory response without decreasing the humoral protective response. The higher IgG1/IgG2a ratio observed supports the possibility of a Th2-dominant response in IFNgamma-deficient animals.
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