Journal
GENES & DEVELOPMENT
Volume 16, Issue 11, Pages 1383-1396Publisher
COLD SPRING HARBOR LAB PRESS
DOI: 10.1101/gad.982902
Keywords
telomere; helicase; telomerase; replication; RRM3; yeast
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Funding
- NIGMS NIH HHS [R37 GM026938, R37 GM26938] Funding Source: Medline
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in wild-type Saccharomyces cerevisiae, replication forks slowed during their passage through telomeric C(1-3)A/TG(1-3) tracts. This slowing was greatly exacerbated in the absence of RRM3, shown here to encode a 5' to 3' DNA helicase. Rrm3p-dependent fork progression was seen at a modified Chromosome VII-L telomere, at the natural X-bearing Chromosome III-L telomere, and at Y'-bearing telomeres. Loss of Rrm3p also resulted in replication fork pausing at specific sites in subtelomeric DNA, such as at inactive replication origins, and at internal tracts of C(1-3)A/TG(1-3) DNA. The ATPase/helicase activity of Rrm3p was required for its role in telomeric and subtelomeric DNA replication. Because Rrm3p was telomere-associated in vivo, it likely has a direct role in telomere replication.
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