Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 68, Issue 6, Pages 3169-3171Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.68.6.3169-3171.2002
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A SYBR Green LightCycler PCR assay using a single primer pair allowed simultaneous detection of stx1 and/or stx2 of Escherichia coli O157:H7. A distinct sequence of the Shiga-like toxin genes was amplified to yield products of 227 and/or 224 bp,: respectively. The two products were distinguished by melting point curve analysis.
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