Journal
CLINICAL IMMUNOLOGY
Volume 103, Issue 3, Pages 260-263Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1006/clim.2001.5212
Keywords
thrombospondin-1; platelet factor 4; TNF alpha-308A; vascular occlusion; juvenile dermatomyositis
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Funding
- NIAMS NIH HHS [R01 AR43978, P60 AR30692] Funding Source: Medline
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Vascular occlusion is more frequent in children with juvenile dermatomyositis (JDM) who have the TNFalpha-308A allele. One of the potent anti-angiogenic factors is thrombospondin-1 (TSP-1). This study investigated the association of the TNFalpha-308A allele with circulating levels of angiogenic mediators, TSP-1, and platelet factor 4 (PF4) using fresh, platelet-poor plasma (PPP). The TNFalpha-308A allele was characterized by PCR amplification and NcoI digestion. Concentrations of TSP-1 and PF4 in PPP from 31 JDM patients and 25 matched pediatric controls were determined by ELISA. The majority of the JDM children with the TNFalpha-308A allele (7/12) produced more TSP-1 than their TNFalpha-308G counterparts (P < 0.05), and their TSP-1 values were inversely related to those for PF4 (P < 0.0006). We conclude that the increased circulating concentrations of TSP-1 associated with the TNFalpha-308A allele suggest that this anti-angiogenic regulator may play a significant role in the augmented vascular occlusion observed in JDM children with this genetic marker. (C) 2002 Elsevier Science (USA).
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