Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 104, Issue 1, Pages 59-67Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0166-0934(02)00039-3
Keywords
TTV; TLMV; real-time PCR; quantitation; circovirus
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There is considerable interest in the possible clinical effects of the human circoviruses TT virus (TTV) and TTV-like mini virus (TLMV). Most people appear to have at least one of these viruses replicating actively in their bodies, thus mere correlation of the presence of virus and disease states are probably less informative than a quantitative analysis of viraemia. Real-time PCR based methods, with either SYBR Green or TaqMan probe, designed to quantitate selectively TTV and TLMV are described. The suggested TaqMan-based protocols were suitable for quantitation of viruses in the range of 10(2)-10(9) copies/ml of sample, and proved, by sequencing of PCR products, to be specific for each of the two viruses. (C) 2002 Elsevier Science B.V. All rights reserved.
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