Journal
PLANT CELL TISSUE AND ORGAN CULTURE
Volume 69, Issue 3, Pages 279-283Publisher
KLUWER ACADEMIC PUBL
DOI: 10.1023/A:1015610011374
Keywords
Asteraceae; micropropagation; shoot bud formation; Tagetes erecta
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Regeneration of whole plants of marigold (Tagetes erecta L.) was achieved by organogenesis using leaf explants. Leaf segments about 0.25 cm(2) were taken from 3-week-old in vitro plantlets and cultured on MS basal medium containing BA with different auxins (NAA, 2,4-D and IAA). The exposure time of the explants on the regeneration medium was tested. The highest values for regeneration were obtained with BA (13.3 muM) and IAA (17.1 muM). Thirteen days was the best time of exposure of the explant to the regeneration medium for shoot induction.
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