Journal
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
Volume 93, Issue 6, Pages 537-542Publisher
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1016/S1389-1723(02)80234-1
Keywords
fusion protein; bioluminescent protein; homogeneous immunoassay; peptide epitope
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A sensitive homogeneous immunoassay is needed in the field of clinical diagnostics. Here we propose a rapid and potentially sensitive homogeneous immunoassay of peptide epitope based on the bioluminescence resonance energy transfer (BRET). A GST-peptide tag fused to the N-terminus of firefly luciferase, and Cy3 or Cy3.5-labeled anti-peptide antibody were prepared to detect BRET from the hybrid luciferase to the fluorolabeled antibody. By measuring the spectral change due to BRET, the amount of c-myc peptide was successfully determined in a short period.
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