Journal
CELL DEATH AND DIFFERENTIATION
Volume 9, Issue 6, Pages 651-660Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.cdd.4401011
Keywords
aging; apoptosis; influenza virus; CD8(+) T cells
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Funding
- NIA NIH HHS [1R01-AG14351, 1R01-AG10057] Funding Source: Medline
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Some studies have reported increased apoptosis in CD8(+) T cells from aged mice. We previously demonstrated diminished virus-specific CD8(+) cytotoxic T lymphocyte (CTL) activity in aged mice in comparison to young mice. The present study investigated the role of apoptosis in age-related influenza virus-specific CD8(+) CTL deficiency. Splenocytes from influenza-primed aged and young mice were stimulated in vitro with virus. The CD8(+) T cell/total lymphocyte ratios correlated with CTL activity and were significantly decreased and increased in aged and young mice, respectively. Fas, FasL, TNF-alpha and TNFR-p55 expression, measured by flow cytometry, ELISA and/or RT-PCR, were significantly elevated in aged mice. Apoptotic CD8(+) T cells (Annexin V binding)were also elevated in aged mice. IL-12 treatment increased CD8(+) CTL activity and IFN-gamma: production but did not affect apoptosis. Thus, apoptosis may contribute to reduced influenza virus-specific CD8(+) T cell frequency, CTL deficiency and increased influenza disease in aging.
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