Journal
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
Volume 1576, Issue 1-2, Pages 176-182Publisher
ELSEVIER
DOI: 10.1016/S0167-4781(02)00286-5
Keywords
CCAAT-binding protein; NF-Y/Hap complex; transcriptional activator; filamentous fungi; protein-protein interaction
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The Saccharomyces cerevisiae CCAAT-binding factor is composed of four subunits Hap2p, Hap3p, Hap4p, and Hap5p. Three subunits, Hap2/3/5p, are required for DNA-binding and Hap4p, is involved in transcriptional activation. Although homologues of Hap2/3/5p (in the case of Aspergillus nidulans; HapB/C/E, respectively) were found in many eukaryotes, no Hap4p homologues have been found except for the other yeast, Kluyveromyces lactis. With the lexA-hap2, -hapB, -hapC, or -hapE fusion gene, we evaluated the ability of interaction between Aspergillus Hap subunits and S. cerevisiae Hap4p subunit in S. cerevisiae. Using the system with lexA-hapB, a gene encoding a novel transcriptional activator, which interacted with the Hap complex, was isolated from A. nidulans and designated hapX. (C) 2002 Elsevier Science B.V. All rights reserved.
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