Journal
JOURNAL OF INFECTIOUS DISEASES
Volume 185, Issue 12, Pages 1736-1744Publisher
UNIV CHICAGO PRESS
DOI: 10.1086/340652
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Funding
- NCI NIH HHS [R03 CA 72929-01, R29 CA 75976-01] Funding Source: Medline
- NCRR NIH HHS [RR 00046] Funding Source: Medline
- NIAID NIH HHS [9P30 AI 50410-04] Funding Source: Medline
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Kaposi's sarcoma-associated herpesvirus (KSHV) is found in Kaposi's sarcoma (KS), multicentric Castleman's disease, and primary effusion lymphomas. To prospectively evaluate KSHV load as a biomarker for KS clinical status and prognosis in a cohort of men with AIDS-related KS, 2 quantitative polymerase chain reaction (PCR) assays were developed and tested to determine KSHV peripheral blood mononuclear cell (PBMC) virus loads. Most patients (13/15) with good-prognosis KS had less than or equal to 1.5 log KSHV copies/10(5) PBMC by both quantitative competitive (QC) and real-time Applied Biosystems (ABI) PCR. Both assays provided 94% specificity for identifying the 16 patients without KS progression during 20 months of follow-up. QC-PCR and ABI-PCR exhibited 100% and 80% levels of diagnostic sensitivity, respectively, for identifying the 5 patients whose KS progressed. Neither dichotomized human immunodeficiency virus loads nor dichotomized CD4 counts predicted either KS progression or KS clinical stage (all positive predictive values <30%). These results are evidence that the quantity of circulating KSHV in KS patients is biologically meaningful and is measurable with sufficient accuracy to provide clinically useful information.
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