4.8 Article

Cardiac nitric oxide synthase 1 regulates basal and β-adrenergic contractility in murine ventricular myocytes

Journal

CIRCULATION
Volume 105, Issue 25, Pages 3011-3016

Publisher

LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1161/01.CIR.0000019516.31040.2D

Keywords

nitric oxide synthase; myocytes; contractility; receptors, adrenergic, beta

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Background-Evidence indicates that myocardial NO production can modulate contractility, but the source of NO remains uncertain. Here, we investigated the role of a type 1 NO synthase isoform (NOS1), which has been recently localized to the cardiac sarcoplasmic reticulum, in the regulation of basal and beta-adrenergic myocardial contraction. Methods and Results-Contraction was assessed in left ventricular myocytes isolated from mice with NOS1 gene disruption (NOS1(-/-) mice) and their littermate controls (NOS1(+/+) mice) at 3 stimulation frequencies (1, 3, and 6 Hz) in basal conditions and during beta-adrenergic stimulation with isoproterenol (2 nmol/L). In addition, we examined the effects of acute specific inhibition of NOS1 with vinyl-L-N-5-(1-imino-3-butenyl)-L-ornithine (L-VNIO, 500 mumol/L). NOS I myocytes exhibited greater contraction at all frequencies (percent cell shortening at 6 Hz, 10.7 +/- 0.92% in NOS1(-/-) myocytes versus 7.21+/-1-0.8% in NOS1(+/+) myocytes; P<0.05) with a flat frequency-contraction relationship. Time to 50% relaxation was increased in NOS1(-/-) myocytes at all frequencies (at 6 Hz, 26.53+/-1.4 ms in NOS1(-/-) myocytes versus 21.27+/-1.3 ms in NOS1(+/+) myocytes; P<0.05). L-VNIO prolonged time to 50% relaxation at all frequencies (at 6 Hz, 21.28+/-1.7 ms in NOS1(+/+) myocytes versus 26.45+/-1.4 ms in NOS1(+/+)+L-VNIO myocytes; P<0.05) but did not significantly increase basal contraction. However, both NOS1(-/-) myocytes and NOS1(+/+) myocytes treated with L-VNIO showed a greatly enhanced contraction in response to β-adrenergic stimulation (percent increase in contraction at 6 Hz, 25.2+/-10.8 in NOS1(+/+) myocytes, 68.2 +/- 11.2 in NOS1(-/-) myocytes, and 65.1+/-13.2 in NOS1(+/+) +L-VNIO myocytes; P<0.05). Conclusions-NOS1 disruption enhances basal contraction and the inotropic response to beta-adrenergic stimulation in murine ventricular myocytes. These findings indicate that cardiac NOS1-derived NO plays a significant role in the autocrine regulation of myocardial contractility.

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