Journal
JOURNAL OF NEUROSCIENCE METHODS
Volume 117, Issue 2, Pages 123-131Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0165-0270(02)00077-8
Keywords
lithography; neuronal growth control; electrophysiology; calcium imaging; immunochemistry; orientation of connectivity
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The use of ordered neuronal networks in vitro is a promising approach to study the development and the activity of small neuronal assemblies. However, in previous attempts, sufficient growth control and physiological maturation of neurons could not be achieved. Here we describe an original protocol in which polylysine patterns confine the adhesion of cellular bodies to prescribed spots and the neuritic growth to thin lines. Hippocampal neurons in these networks are maintained healthy in serum free medium up to 5 weeks in vitro. Electrophysiology and immunochemistry show that neurons exhibit mature excitatory and inhibitory synapses and calcium imaging reveals spontaneous activity of neurons in isolated networks. We demonstrate that neurons in these geometrical networks form functional synapses preferentially to their first neighbors. We have, therefore, established a simple and robust protocol to constrain both the location of neuronal cell bodies and their pattern of connectivity. Moreover, the long term maintenance of the geometry and the physiology of the networks raises the possibility of new applications for systematic screening of pharmacological agents and for electronic to neuron devices. (C) 2002 Elsevier Science B.V. All rights reserved.
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