Sequences of the genomic DNAs encoding the S2, S9, S10, and S23 alleles from almond, Prunus dulcis

Partial genomic sequences of the S-alleles S2, S9, and S10, identified in genomic DNA selected from Prunus dulcis 'Cristomorto' (S1S2), 'Anxaneta' (S2S9), 'Primorskiy' (S5S9), and 'Gabaix' (S5S10), were found to contain introns of 562, 1,343, and similar to705 bp, and partial exons of 537, 486, and 495 bp respectively. In addition, another allele, characterized as S23, was identified in the two Australian cultivars; Johnston's Prolific and Pierce. This allele had an intron size of 662 bp and a partial exon size of 489 bp, and may have been introduced into Australia in the Spanish cultivar Ramillete (S6S23). The exon/intron splice junction sites of all alleles followed the GT/AG consensus sequence rule, and the sequences were found to be highly conserved.