Journal
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
Volume 122, Issue 2, Pages 201-210Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0166-6851(02)00103-2
Keywords
Plasmodium falciparum; placenta; erythrocyte; antigenic variation; adhesion; transcription
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Funding
- NIAID NIH HHS [R01 AI48654, R01 AI43680] Funding Source: Medline
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Adhesion to chondroitin sulfate A (CSA), a distinguishing feature of malaria parasites obtained from the human placenta, might be mediated by the Duffy-binding-like (DBL) gamma domain of the variant surface antigen Plasmodium falciparum erythrocyte membrane protein-1 (PfEMP1). We studied transcription of var genes (that encode PfEMP1) in placental parasites by amplifying and sequencing DBLgamma fragments from genomic DNA and cDNA of Field isolates collected in western Kenya. We amplified DBLgamma fragments with divergent sequences from individual isolates by using various sequence-specific or degenerate primers. Transcripts detected with degenerate primers clustered phylogenetically within two DBLgamma subtypes with homology to chr5_l.gen_150 or FCR3.varCSA. Interestingly, the DBLalpha encoded by chr5_l.gen_150 was recently found to be commonly expressed by placental isolates from Malawi (Mol. Biochem. Parasitol. 185 (2002) 1207). The findings are consistent with earlier serologic evidence that surface antigens of placental parasites have conserved features, and Suggest that vaccines based on DBLgamma may only need to target a limited number of variants. (C) 2002 Elsevier Science B.V. All rights reserved.
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