Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 68, Issue 7, Pages 3651-3654Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.68.7.3651-3654.2002
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A raw-starch-digesting amylase (RSDA) gene from a Cytophaga sp. was cloned and sequenced. The predicted protein product contained 519 amino acids and had high amino acid identity to alpha-amylases from three Bacillus species. Only one of the Bacillus alpha-amylases has raw-starch-digesting capability, however. The RSDA, expressed in Escherichia coli, had properties similar to those of the enzyme purified from the Cytophaga sp.
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