Association of plasma soluble α-klotho with pro-endothelin-1 in patients with type 2 diabetes

Objectives: To study the relationship between plasma soluble klotho (sKlotho) and pro-endothelin-1 (proET-1) in patients with type 2 diabetes (T2DM). Subjects and methods: In this cross-sectional study, we recruited 175 T2DM subjects and 56 non-diabetic controls. Plasma sKlotho, proET-1 and extracellular superoxide dismutase (SOD) were measured by ELISA and ILMA, respectively. Results: Plasma sKlotho level in patients with T2DM was lower compared to that in non-diabetic controls (416.8 +/- 148.1 vs. 494.6 +/- 134.3 pg/ml, p = 0.001) and showed significant interaction with diabetes status in its association with proET-1. Plasma sKlotho was inversely correlated with proET-1 in T2DM (Rho = -0.410, p < 0.0001) but not in non-diabetic controls (Rho = 0.091, p = 0.505). Multivariable linear regression models revealed that sKlotho was independently associated with proET-1 after adjustment for renal filtration function, albuminuria, diabetes duration, HbA1c, systolic and diastolic blood pressure. Conclusions: Plasma sKlotho was associated with proET-1 independent of renal function in patients with T2DM. (C) 2014 Elsevier Ireland Ltd. All rights reserved.