Journal
ATHEROSCLEROSIS
Volume 208, Issue 1, Pages 75-82Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.atherosclerosis.2009.06.028
Keywords
ABC transporter; Reverse cholesterol transport; Macrophage; Vascular endothelial cells; Smooth muscle cells
Funding
- National Health and Medical Research Council of Australia
- Heart Foundation of Australia
- University of New SouthWales
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Objective: To evaluate the expression of two ABCG1 isoforms that differ in the presence or absence of a 12 amino acid (AA) peptide between the ABC cassette and the transmembrane region, termed ABCG1(+ 12) and ABCG1(-12), respectively, in human vascular cells and tissues. Methods and results: mRNA for both isoforms was expressed in human macrophages, vascular endothelial and smooth muscle cells as well as whole human spleen, lung, liver and brain tissue. However, ABCG1(+ 12) was not expressed in mouse tissues. 2D gel electrophoresis of ABCG1 protein indicated that both protein isoforms were expressed in human macrophages. Furthermore the half-lives of the two ABCG1 protein isoforms, stably expressed in CHOK1 cells, measured under basal conditions were different, suggesting the presence of a degradation or stabilising signal in or near the 12AA region of ABCG1(+ 12). Conclusion: ABCG1(+ 12) is an isoform of ABCG1 exclusively expressed in human cells at the RNA and protein level. As ABCG1(+ 12) is not expressed in mice, although mouse models are widely used to elucidate the function of ABCG1, further investigations into the importance of this human ABCG1 isoform are warranted. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
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