Lipogenesis in the basidiomycetes Pleurotus ostreatus and Flammulina velutipes cultivated on different media

The compositions of free fatty acids (FA) in the mycelia of oyster cap (Pleurotus ostreatus (Jacq. ex Fr.) Kumm.) and honey mushroom (Flammulina velutipes (Curt. ex Fr.) Sing.) and the effect of mycelium cultivation conditions on the composition and proportions of individual FA were investigated. Palmitic and linoleic acids were found to be major acids produced by P ostreatus growing on solid agar medium and in a submerged culture with a synthetic medium. The composition of minor FA in P ostreatus was dependent on cultivation conditions. Surface cultivation of its mycelium yielded pentadecanoic, octadecenoic, and stearic acids. Submerged cultivation additionally yielded undecanoic, myristic, hexadecenoic, and lignoceric acids. The composition of free FA in F velutipes showed no significant differences from that of P ostreatus. Variation in the C/N ratio in the cultivation medium affected both the FA composition in P ostreatus and E velutipes and the relationship between saturated and unsaturated FA.