Journal
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 102, Issue -, Pages 109-118Publisher
HUMANA PRESS INC
DOI: 10.1385/ABAB:102-103:1-6:109
Keywords
neem hulls; white-rot fungi; solid-state fermentation; ligninolytic enzymes; lignocellulosic waste
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Lignocellulosic wastes such as neem hull, wheat bran, and sugarcane bagasse, available in abundance, are excellent substrates for the production of ligninolytic enzymes under solid-state fermentation by white-rot fungi. A ligninolytic enzyme system with high activity showing enhanced decomposition was obtained by cocultivation of Pleurotus ostreatus and Phanerochaete chrysosporium on combinations of lignocellulosic waste. Among the various substrate combinations examined, neem hull and wheat bran wastes gave the highest ligninolytic activity. A maximum production of laccase of 772 U/g and manganese peroxidase of 982 U/g was obtained on d 20 and lignin peroxidase of 656 U/g on d 25 at 28 +/- 10degreesC under solid-state fermentation. All three enzymes thus obtained were partially purified by acetone fractionation and were exploited for decolorizing different types of acid and reactive dyes.
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