Journal
METABOLIC ENGINEERING
Volume 4, Issue 3, Pages 193-201Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1006/mben.2002.0225
Keywords
isoprenoids; lycopene; neurosporaxanthin; Neurospora crassa; metabolic engineering
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Neurospora crassa, a filamentous fungus, naturally produces the carotenoids lycopene and neurosporaxanthin. To increase the carbon flux through the carotenoid biosynthetic pathway, the 1658-bp region of the HMG1 gene encoding the catalytic domain (cHMG1) of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase of Saccharomyces cerevisiae was expressed in N. crassa under control of the strong, constitutive glyceraldehyde-3-phosphate dehydrogenase (GPD) promoter and the inducible alcohol dehydrogenase (alcA) promoter. Overexpressing cHMG1 under control of the GPD promoter increased lycopene and neurosporaxanthin production 6- and 1.5-fold, respectively, relative to the wild-type strain. Overexpression of cHMG1 under control of the alcA promoter increased production of lycopene and neurosporaxanthin 3- and 2-fold, respectively. (C) 2002 Elsevier Science (USA).
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