Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 373, Issue 6, Pages 332-343Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-002-1288-9
Keywords
review; capillary electrophoresis; laser-induced fluorescence; wavelength-resolved fluorescence
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Capillary electrophoresis (CE) enables rapid separations with high separation efficiency and compatibility with small sample volumes. Laser-induced fluorescence detection can result in extremely low limits of detection in CE. Single-channel fluorescence detection, however, furnishes little qualitative information about a species being detected, except for its CE migration time. Use of multidimensional information often enables unambiguous identification of analytes. Combination of CE with information-rich wavelength-resolved fluorescence detection is analogous with ultraviolet-visible diode-array detection and furnishes both qualitative and quantitative chemical information about target species. This review discusses recent advances in wavelength-resolved laser-induced fluorescence detection coupled with CE, with an emphasis on instrument design.
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