Journal
LIFE SCIENCES
Volume 71, Issue 7, Pages 813-817Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0024-3205(02)01732-0
Keywords
neuronal nitric oxide synthase; neuroblastoma cells; western blot analysis
Funding
- NINDS NIH HHS [NS25743] Funding Source: Medline
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Several neuronal cell lines were screened for endogenous expression of neuronal nitric oxide synthase (nNOS) protein using Western blot analysis. Detectable levels of the nNOS protein were evident in the SK-N-SH, SH-SY5Y, and NIE-115 neuroblastoma cell lines, as well as the NG108-15 neuroblastoma X glioma hybrid. Only trace amounts were visible in Neuro2A human neuroblastoma cells. The presence of endogenously expressed nNOS in these cells may allow for the study of the interaction between nNOS and the endogenous receptor systems expressed in the same cells. (C) 2002 Elsevier Science Inc. All rights reserved.
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