Journal
ORGANIC LETTERS
Volume 4, Issue 14, Pages 2305-2308Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ol026043x
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- NIGMS NIH HHS [GM 58447] Funding Source: Medline
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[GRAPHICS] A fluorescent deoxyuridine analogue is sensitive to the polarity of its environment and exhibits a distinct emission profile in single- vs double-stranded oligonucleotides. Emission-monitored denaturation curves of internally modified dU(phen) duplexes are characteristic of the base opposite dU(phen) and distinguish between perfect and mismatched complementary oligonucleotides.
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