Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 277, Issue 28, Pages 25070-25081Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M202683200
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Deamination of LPSs from Klebsiella pneumoniae released O-chain polysaccharides together with a fragment of the core oligosaccharide. The structures of the products from serotypes 01, O2a, O2a,c, 03, 04, 05, and 012 were determined by NMR spectroscopy and chemical methods, identifying the linkage region between the 0 antigens and the core as well as novel residues at the non-reducing ends of the polysaccharides. All serotypes had an identical linkage between the 0 chain and core. [GRAPHICS] where L-glycero-D-manno-heptose is non-stoichiometric. Analysis of the LPSs from a waaL (O-polysaccharide ligase-deficient) mutant showed that the beta-GlcNAc in the linkage region is derived from the O-chain biosynthesis pathway, and the alpha-3-deoxy-D-manno-octulosonic acid represents the core residue to which the 0 chain is ligated. Consistent with the common ligation site, the WaaL proteins from these serotypes are essentially identical. Polysaccharides from serotypes 01, O2a, and O2a,c contain no special groups at the non-reducing end, whereas polysaccharides from serotypes 03,04,05, and 012 have residues at the non-reducing end that are not found in the polymer repeating units.
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