Journal
ANALYTICAL CHEMISTRY
Volume 74, Issue 14, Pages 3378-3385Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac025551h
Keywords
-
Categories
Funding
- NHGRI NIH HHS [R01-HG01406] Funding Source: Medline
Ask authors/readers for more resources
In this paper, we demonstrate immobilization and stretching of single lambda-phage DNA molecules within microfluidic systems using ac fields. We present a novel thiol-on-gold-based immobilization technique for fixing one specific end (T end) of a DNA molecule onto a gold electrode. A polymer-enhanced medium (similar to3.75 wt % linear polyacrylamide in Tris-HCl) is used to obtain fully stretched configurations (21 mum) of fluorescently stained lambda-DNA molecules. We also present an optimized microelectrode design with pointed electrodes and an electrode spacing of 20 mum for stretching DNA molecules with an ac field (1 MHz, 3 x 10(5) V/m). Finally, using these techniques, we immobilize a single DNA molecule at one electrode Edge, stretch the molecule, and fix the other end at an adjacent electrode edge, forming a bridge between two electrodes within a microfabricated device.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available