The 1.62 Å structure of Thermoascus aurantiacus endoglucanase:: completing the structural picture of subfamilies in glycoside hydrolase family 5

The crystal structure of Thermoascus aurantiacus endoglucanase (Ce15A), I family 5 glycoside hydrolase, has been determined to 1.62 Angstrom resolution by multiple isomorphous replacement with anomalous scattering. It is the first report of a structure in the subfamily to which Ce15A belongs. Ce15A consists solely of a catalytic module with compact eight-fold beta/alpha barrel architecture. The length of the tryptophan-rich substrate binding groove suggests the presence of substrate binding sub-sites -4 to +3. Structural comparison shows that two glycines are completely conserved in the family, in addition to the two catalytic glutamates and six other conserved residues previously identified. Gly 44 in particular is part of a type IV C-terminal helix capping motif, whose disruption is likely to affect the position of an essential conserved arginine. One aromatic residue (Trp 170 in Ce15A), not conserved in term of sequence, is nonetheless spatially conserved in the substrate binding groove. Its role might be to force the bend that occurs in the polysaccharide chain on binding, thus favoring substrate distortion at subsite -1. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.