Journal
NEUROREPORT
Volume 13, Issue 10, Pages 1263-1266Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/00001756-200207190-00009
Keywords
BAT; Ca2+ indicator; chromophore; laser; MALC; microinjection
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Microinjection is one of the most useful and important methods in cellular neurobiology. However, direct insertion and retraction of a capillary produce physical stresses inside cells that make it difficult to apply on small and fragile cells, especially the central nervous system (CNS) neurons. In this study, we developed a novel method called MALC (microinjection method assisted with laser activated chromophore) to avoid this disadvantage of the conventional method. In MALC, a capillary is just attached on cell surface, and combination of a newly developed photosensitizer with a laser pointing system makes the plasma membrane permeable to molecules. This novel method can expand the application of the microinjection method and realize molecular manipulations inside CNS neurons.
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