Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 963, Issue 1-2, Pages 125-136Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0021-9673(02)00220-0
Keywords
water analysis; environmental analysis; vegetables; food analysis; immunoassays; alachlor; pesticides
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The use of a rapid and specific (cross-reactivity<4%) enzyme-linked immunosorbent assay (ELISA) for the determination of alachlor residues in water and vegetable samples is addressed. The analytical method consists of a fast extraction procedure followed by an optimised ELISA. The detection limit was 0.44 mug l(-1), with a linear range from 0.89 to 143.2 mug l(-1). For alachlor extraction from water samples, different solid-phase cartridges (C-18, Ph, C-8 and C-2) were assayed using MeOH as eluent. Extracts were diluted (1:4) with distilled water before ELISA. This procedure gave recoveries close to 100% with RSDs<14%. For vegetable samples, alachlor was extracted directly with MeOH and the extracts diluted 1:40 (v/v) with saline buffer prior to ELISA. The results obtained by the proposed procedure correlate well with the reference method (multiresidue extraction-GC-MS) for vegetable samples (r>0.85). (C) 2002 Elsevier Science B.V. All rights reserved.
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