Determination of nicotinamide and 4-aminobenzoic acid in pharmaceutical preparation by LC

A rapid, precise and time saving high performance liquid chromatographic method has been developed and validated for the simultaneous determination of nicotinamide and 4-aminobenzoic acid in pharmaceutical preparation. The method involves isocratic elution of mobile phase through column in a reverse phase chromatography with UV detection at 254 nm. The ranges of quantification for nicotinamide and 4-aminobenzoic acid were 11-34 mug ml(-1) and 37-113 mug ml(-1), respectively. Investigating specificity, linearity, precision, accuracy, robustness and ruggedness performed the validation of the method. (C) 2002 Elsevier Science B.V. All rights reserved.