Journal
JOURNAL OF INORGANIC BIOCHEMISTRY
Volume 91, Issue 1, Pages 306-311Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/S0162-0134(02)00362-8
Keywords
cisplatin; transplatin; protein binding; electrospray ionization mass spectrometry (ESI-MS)
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In this manuscript we report on the interactions of cis-DDP (cisplatin, cis-diamminedichloroplatinum(II)) and trans-DDP (transplatin, trans-diamminedichloroplatinum(II)) with two model proteins, ubiquitin (Ub) and horse heart myoglobin (Mb), and attempt to answer the question whether proteins that have methionine-Pt adducts can transfer the platinum to biological nucleophiles and particularly to DNA. Our study shows that cisplatin and transplatin form different adducts with ubiquitin: transplatin forms one major adduct, trans[Pt(Ub)(NH3)(2)Cl], while cisplatin forms four distinct adducts, [Pt(Ub)(NH3)(2)Cl], [Pt(Ub)(NH3)(2)(H2O)], [Pt(Ub)(NH3)(2)], and [Pt(Ub)(NH3)]. When binding ubiquitin, Metl is the preferred binding site of cisplatin, but not of transplatin. Cisplatin binds faster than transplatin to both ubiquitin and horse heart myoglobin. Both cisplatin and transplatin adducts form stable ternary adducts when reacted with 5'-guanosine monophosphate (5'-GMP) or a tetranucleotide. No transfer of the Pt moiety from the proteins to the nucleotides was observed. Glutathione efficiently removes the platinum from preformed adducts of both cisplatin and transplatin with ubiquitin. (C) 2002 Elsevier Science Inc. All rights reserved.
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