A method for the incorporation of ovalbumin into immune stimulating complexes prepared by the hydration method

This study describes the development of a method for the incorporation of fluorescently labelled ovalbumin (FITC-OVA) into immune stimulating complexes (ISCOMs) prepared by the hydration method, Conjugation of palmitic acid vas performed to fluorescently labelled OVA (pFITC-OVA) or to non-labelled OVA. with subsequent conjugation of FITC to the resulting palmitified OVA (FITC-pOVA). Both pFITC-OVA and FITC-pOVA. but not FITC-OVA, Could be incorporated into ISCOMs and other non-liposomal colloidal structures. The degree of incorporation of pFITC-OVA or FITC-pOVA in non-liposomal colloidal particles reaches a maximum, if ISCOMs are the predominant colloids in the system. (C) 2002 Elsevier Science B.V. All rights reserved.